Generator

Part:BBa_K4462110:Design

Designed by: Kredai Raaman   Group: iGEM22_IISc-Bengaluru   (2022-09-30)


pCAM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1709
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2062
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Assembly

References

BRENDA Enzyme Database (brenda-enzymes.org) Camphor 5-monooxygenase - Wikipedia Alagappan, G., & Cowan, R. M. (2004). Effect of temperature and dissolved oxygen on the growth kinetics of pseudomonas putida F1 growing on benzene and toluene. Chemosphere, 54(8), 1255–1265. https://doi.org/10.1016/j.chemosphere.2003.09.013 Oremland, R. S., Lonergan, D. J., Culbertson, C. W., & Lovley, D. R. (1996). Microbial degradation of hydrochlorofluorocarbons (chcl2f and CHCl2CF3) in soils and sediments. Applied and Environmental Microbiology, 62(5), 1818–1821. https://doi.org/10.1128/aem.62.5.1818-1821.1996 Zylstra GJ, Gibson DT. Toluene degradation by Pseudomonas putida F1. Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli. J Biol Chem. 1989 Sep 5;264(25):14940-6. PMID: 2670929.